Tetrapentylammonium block of chloramine-T and veratridine modified rat brain type IIA sodium channels
نویسندگان
چکیده
منابع مشابه
Proton Block of Rat Brain Sodium Channels
The acid titration function of bilayer-incorporated batrachotoxin (BTX)-modified sodium channels was examined in experiments in which the pH was decreased symmetrically, on both sides of the membrane, or asymmetrically, on only one side. In an a t tempt to minimize interpretat ional ambiguities, the experiments were done in 1.0 M NaCI (buffered to the appropr ia te pH) with channels incorporate...
متن کاملVeratridine modifies open sodium channels
The state dependence of Na channel modification by the alkaloid neurotoxin veratridine was investigated with single-channel and whole-cell voltage-clamp recording in neuroblastoma cells. Several tests of whole-cell Na current behavior in the presence of veratridine supported the hypothesis that Na channels must be open in order to undergo modification by the neurotoxin. Modification was use dep...
متن کاملSome properties of sodium channels in neuroblastoma cells modified with scorpion toxin and chloramine-T. Single channel measurements.
Currents through sodium channels of neuroblastoma cells were measured using patch technique in outside-out configuration. Scorpion toxin (ScTX) produced 3 to 4 fold prolongation of mean open time and increased number of reopenings. The mean open times showed slow fluctuations around some average values. The distribution of channel open times for ScTX-modified channels required more than one exp...
متن کاملRapid and slow gating of veratridine-modified sodium channels in frog myelinated nerve
The properties of voltage-dependent Na channels modified by veratridine (VTD) were studied in voltage-clamped nodes of Ranvier of the frog Rana pipiens. Two modes of gating of VTD-modified channels are described. The first, occurring on a time scale of milliseconds, is shown to be the transition of channels between a modified resting state and a modified open state. There are important qualitat...
متن کاملGrayanotoxin-I-modified eel electroplax sodium channels. Correlation with batrachotoxin and veratridine modifications
To probe the structure-function relationships of voltage-dependent sodium channels, we have been examining the mechanisms of channel modification by batrachotoxin (BTX), veratridine (VTD), and grayanotoxin-I (GTX), investigating the unifying mechanisms that underlie the diverse modifications of this class of neurotoxins. In this paper, highly purified sodium channel polypeptides from the electr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 2001
ISSN: 0007-1188
DOI: 10.1038/sj.bjp.0703973